The structure of Aerobacter aerogenes capsular polysaccharide.
نویسندگان
چکیده
A capsular polysaccharide produced by Aerobacter aerogenes has been purified and shown to consist of galactose, mannose, and glucuronic acid in a molar ratio of 2: 1: 1, respectively. Partial acid hydrolysis liberated an aldobiouronic acid identified as 2-0-P-D-glucuronosyl-D-mannose. Analysis of capsular polysaccharide by Smith periodate degradation further indicated that all of the galactose residues are linked through C-3, all of the mannose residues are linked through both C-2 and C-3, and all of the glucuronic acid is at terminal, nonreducing positions. Digestion of capsular polysaccharide with a specific phageinduced polysaccharide depolymerase resulted in its nearly quantitative conversion to two limit oligosaccharides, termed A and B. Partial acid hydrolysis, methylation analysis, and periodate degradation indicated that A is the tetrasaccharide, Gal-l --* 3-(GlcUA-1 -+ 2)-Man-l -+ 3-Gal. B was characterized as an octasaccharide composed of two units of A by the following procedure: Smith periodate degradation of B
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 246 18 شماره
صفحات -
تاریخ انتشار 1971